Rabbits  (Oryctolagus  cuniculus)  are  valuable  as  laboratory  animals  and  farm  assets  due  to  their adaptability and rapid reproduction. They can host ticks that feed on blood and transmit various pathogens. Ticks and blood samples were collected from the AFU livestock farm from September 2023 to December 2023. The size of morphologic features was recorded, and the species was identified using taxonomic keys. An automatic hematology analyzer was used for hematological  analysis  of  blood  samples.  A  Giemsa-stained  blood  smear  was  observed  with  1000x  magnification  using  a  binocular  compound  microscope  for  haemoparasites.  About 32  tick  specimens  were  collected  from  16  rabbits,  comprising  37.50%  adult  males,  25.00%  adult females, 25.00% larvae, and 12.50% nymphs. All adults and nymphs were identified as Haemaphysalis howletti. The rabbits had a higher prevalence of ticks during early autumn (100%) than in late autumn (0%). There was a significant decrease in Total Erythrocyte Count (TEC), Hemoglobin (HGB), Hematocrit (HCT), and Platelet count (PLT), whereas an increase in Total Leucocyte Count (TLC) and granulocyte count occurred during the tick-infested state in  rabbits  (P<0.05).  In  addition,  the  overall  prevalence  of  Anaplasma spp.  was  31.25%  in  rabbits. Here, tick infestation showed a major role in hematological alterations and reservoirs for tick-borne pathogens. Thus, ultimately affects the health of rabbits, adding potential risk of zoonosis, and a challenge to public health.